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Addgene

pcU6.3_gRNA_c1
(Plasmid #221381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcU6.3
  • Backbone size (bp) 3518
  • Modifications to backbone
    10x capture sequence 1 insertion
  • Vector type
    CRISPR
  • Promoter G. gallus U6.3

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BsmBI cut sites flanking the gRNA spacer. gRNA to be inserted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcU6.3_gRNA_c1 was a gift from James Briscoe & Tatjana Sauka-Spengler (Addgene plasmid # 221381 ; http://n2t.net/addgene:221381 ; RRID:Addgene_221381)
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