pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
(Plasmid
#221364)
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PurposeMammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepTipi2.1
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Backbone manufacturerInstitute for Protein Innovation (IPI)
- Backbone size w/o insert (bp) 3873
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
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SpeciesO. cuniculus (rabbit)
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Insert Size (bp)654
- Promoter CMV
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that both plasmids, heavy chain (Plasmid 221363) and light chain (Plasmid 221364), are required to make the antibody.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa was a gift from Institute for Protein Innovation (IPI) (Addgene plasmid # 221364 ; http://n2t.net/addgene:221364 ; RRID:Addgene_221364)