pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
(Plasmid #221357)

• Purpose: Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.
• Depositing Lab: Institute for Protein Innovation (IPI)
• Publication: Institute for Protein Innovation (IPI) Epitope Tag Antibody Plasmids (unpublished)

Backbone

• Vector backbone: pTipi2.1
• Backbone manufacturer: Institute for Protein Innovation (IPI)
• Backbone size w/o insert (bp): 3876
• Vector type: Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
• Species: O. cuniculus (rabbit)
• Insert Size (bp): 1326
• Promoter: CMV

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note that both plasmids, heavy chain (Plasmid 221357) and light chain (Plasmid 221358), are required to make the antibody.