HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro
(Plasmid #22135)

• Depositing Lab: William Kaelin
• Publication: Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Backbone

• Vector backbone: pBabe-Puro
• Backbone size w/o insert (bp): 5188
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha BL21
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hypoxia inducible factor 2 alpha
• Alt name: HIF2a
• Alt name: EPAS1
• Species: H. sapiens (human)
• Insert Size (bp): 2655
• Mutation: cDNA changed amino acids from wt: Proline 405 to Alanine Proline 531 to Alanine mutation in bHLH region changed amino acids 24-29 from wt: RCRRSK to ACAASA
• GenBank ID: NM_001430
• Entrez Gene: EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (destroyed during cloning)
• 5′ sequencing primer: pBabe-5'
• 3′ sequencing primer: pBabe-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry