pET28a-LflChmH
(Plasmid
#221344)
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PurposeExpress LflChmH in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechmH
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SpeciesLentzea flaviverrucosa DSM 44664
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCodon optimized and synthesized by Twist Biosciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Codon optimized for expression in E. coli.
Please visit https://www.biorxiv.org/content/10.1101/2024.01.24.577118v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-LflChmH was a gift from Emily Balskus (Addgene plasmid # 221344 ; http://n2t.net/addgene:221344 ; RRID:Addgene_221344) -
For your References section:
Elucidation of Chalkophomycin Biosynthesis Reveals N-Hydroxypyrrole-Forming Enzymes. Crooke AM, Chand AK, Cui Z, Balskus EP. J Am Chem Soc. 2024 Jun 12;146(23):16268-16280. doi: 10.1021/jacs.4c04712. Epub 2024 May 29. 10.1021/jacs.4c04712 PubMed 38810110
