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Addgene

pET28a-LflChmG
(Plasmid #221343)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221343 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    chmG
  • Species
    Lentzea flaviverrucosa DSM 44664
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized and synthesized by Twist Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for expression in E. coli.

Please visit https://www.biorxiv.org/content/10.1101/2024.01.24.577118v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-LflChmG was a gift from Emily Balskus (Addgene plasmid # 221343 ; http://n2t.net/addgene:221343 ; RRID:Addgene_221343)

  • For your References section:

    Elucidation of Chalkophomycin Biosynthesis Reveals N-Hydroxypyrrole-Forming Enzymes. Crooke AM, Chand AK, Cui Z, Balskus EP. J Am Chem Soc. 2024 Jun 12;146(23):16268-16280. doi: 10.1021/jacs.4c04712. Epub 2024 May 29. 10.1021/jacs.4c04712 PubMed 38810110
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