Skip to main content
Addgene

pET28a-FKBP-MAVS(pLxIS)
(Plasmid #221335)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAVS pLxIS domain
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mavs (a.k.a. D430028G21Rik, IPS-1, Visa, cardif)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-4xFKBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-FKBP-MAVS(pLxIS) was a gift from Jonathan Kagan (Addgene plasmid # 221335 ; http://n2t.net/addgene:221335 ; RRID:Addgene_221335)

  • For your References section:

    pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism. Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC. Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25. 10.1016/j.molcel.2024.05.030 PubMed 38925114
Related items: