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pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)
(Plasmid #221330)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-TRE3G
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-IRSp53 and HA-Rac1(G12V)
  • Species
    M. musculus (mouse)
  • Mutation
    G12V
  • Entrez Gene
    Baiap2 (a.k.a. IRSp53)
  • Entrez Gene
    Rac1 (a.k.a. D5Ertd559e)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V) was a gift from Jonathan Kagan (Addgene plasmid # 221330 ; http://n2t.net/addgene:221330 ; RRID:Addgene_221330)

  • For your References section:

    pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism. Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC. Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25. 10.1016/j.molcel.2024.05.030 PubMed 38925114
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