pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
(Plasmid
#221329)
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PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRetroX-TRE3G
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V) was a gift from Jonathan Kagan (Addgene plasmid # 221329 ; http://n2t.net/addgene:221329 ; RRID:Addgene_221329) -
For your References section:
pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism. Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC. Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25. 10.1016/j.molcel.2024.05.030 PubMed 38925114