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pRetroX-TRE3G-IRSp53 P428L
(Plasmid #221321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-TRE3G
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRSp53
  • Species
    M. musculus (mouse)
  • Mutation
    P428L
  • Entrez Gene
    Baiap2 (a.k.a. IRSp53)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a 35bp deletion in the PGK promoter. This deletion is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-TRE3G-IRSp53 P428L was a gift from Jonathan Kagan (Addgene plasmid # 221321 ; http://n2t.net/addgene:221321 ; RRID:Addgene_221321)

  • For your References section:

    pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism. Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC. Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25. 10.1016/j.molcel.2024.05.030 PubMed 38925114
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