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Addgene

ATF4.18: mATF4 (CHOP11/cATF)-GST
(Plasmid #22130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATF4/CHOP11
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

record is incomplete on this issue but the preponderance of the evidence suggests that this was the immunogen used to produce the antiserum referred to as "CHOP 11 FL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATF4.18: mATF4 (CHOP11/cATF)-GST was a gift from David Ron (Addgene plasmid # 22130 ; http://n2t.net/addgene:22130 ; RRID:Addgene_22130)
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