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Addgene

pMSCV-FKBP-IRSp53(trunc C4)
(Plasmid #221276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRSp53 C-terminal truncation 4 (247-295)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Baiap2 (a.k.a. IRSp53)
  • Tag / Fusion Protein
    • FLAG-4xFKBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-FKBP-IRSp53(trunc C4) was a gift from Jonathan Kagan (Addgene plasmid # 221276 ; http://n2t.net/addgene:221276 ; RRID:Addgene_221276)
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