pFLAG-6a-Ataxin3Q22-C14A
(Plasmid #22127)

• Depositing Lab: Henry Paulson
• Publication: Henry Paulson's unpublished plasmids (unpublished)

Backbone

• Vector backbone: pFLAG-CMV-6a
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ataxin 3
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: C14A
• Entrez Gene: ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry