Skip to main content
Addgene

tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
(Plasmid #221235)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    FUGW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal lab identifier DC849.

Please visit https://doi.org/10.1101/2024.02.01.577593 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA was a gift from Fei Chen (Addgene plasmid # 221235 ; http://n2t.net/addgene:221235 ; RRID:Addgene_221235)

  • For your References section:

    Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes. Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F. bioRxiv 2024.02.01.577593 10.1101/2024.02.01.577593
Related items:
Commonly requested with: