tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
(Plasmid
#221235)
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PurposeTet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUGW
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal lab identifier DC849.
Please visit https://doi.org/10.1101/2024.02.01.577593 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA was a gift from Fei Chen (Addgene plasmid # 221235 ; http://n2t.net/addgene:221235 ; RRID:Addgene_221235) -
For your References section:
Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes. Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F. bioRxiv 2024.02.01.577593 10.1101/2024.02.01.577593
