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Addgene

U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
(Plasmid #221233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX461
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9(D10A)-P2A-EGFP
  • gRNA/shRNA sequence
    GGCCCAGACTGAGCACGTGA
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal lab identifier DC562.

Please visit https://doi.org/10.1101/2024.02.01.577593 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP was a gift from Fei Chen (Addgene plasmid # 221233 ; http://n2t.net/addgene:221233 ; RRID:Addgene_221233)

  • For your References section:

    Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes. Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Chao YK, Navarro Torres A, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F. Science. 2024 Oct 11;386(6718):eadn5876. doi: 10.1126/science.adn5876. Epub 2024 Oct 11. 10.1126/science.adn5876 PubMed 39388570
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