U6-BbsI-GGsite-mCherry-Puro
(Plasmid
#221232)
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Purpose(Empty Backbone) Cloning vector for sgRNA with mCherry and Puro
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Vector contains sgRNA scaffold and mCherry-Puro.
Internal lab identifier DC341.
Please visit https://doi.org/10.1101/2024.02.01.577593 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U6-BbsI-GGsite-mCherry-Puro was a gift from Fei Chen (Addgene plasmid # 221232 ; http://n2t.net/addgene:221232 ; RRID:Addgene_221232) -
For your References section:
Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes. Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F. bioRxiv 2024.02.01.577593 10.1101/2024.02.01.577593