pVETL-Cmcs-Flag-Ataxin3Q80-C14A
(Plasmid #22121)

• Depositing Lab: Henry Paulson
• Publication: Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-58.

Backbone

• Vector backbone: pVETL-Cmcs
• Backbone size w/o insert (bp): 5756
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ataxin 3
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: C14A
• Entrez Gene: ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: ApaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pVETL Cmcs vector map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry