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pET3a-XlH3[ChEx]
(Plasmid #221200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21-DE3 Rosetta Star cells can be used for expression. Induce with IPTG at OD of 0.5 and harvest ~3.5 hours post-induction.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Xl-H3[ChEx]
  • Alt name
    Histone H3 [ChEx] fusion
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    381

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a-XlH3[ChEx] was a gift from Gregory Bowman (Addgene plasmid # 221200 ; http://n2t.net/addgene:221200 ; RRID:Addgene_221200)

  • For your References section:

    Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state. Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP. Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16. 10.1038/s41594-021-00719-x PubMed 35173352
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