pDEST17-ScChd1[Y137A]
(Plasmid #221198)

• Purpose: ScChd1-aa118-1274 chromatin remodeler with Y137A mutation
• Depositing Lab: Gregory Bowman
• Publication: Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16.

Backbone

• Vector backbone: pDEST17
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21-DE3 Rosetta Star cells can be used for expression. grow at 37˚C then shift to 16˚C at OD600 one doubling before inducing with IPTG
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ScChd1 (aa118-1274)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3468
• Mutation: Y137A
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-tag (Precission protease cleavable) (N terminal on backbone)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: 5’ ct aat cgt caa aac aaa acg gta aat GCt aac atc gat tat agc gat gat gac ctc
• 3′ sequencing primer: 5’ gaggtcatcatcgctataatcgatgttaGCatttaccgttttgttttgacgattag

Resource Information

• Supplemental Documents:
  • ScChd1(142-1274)-Y137A mutation
• A portion of this plasmid was derived from a plasmid made by: full length yeast Chd1 was a gift from Paul Kaufman

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry