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pDEST17-ScChd1[R126A/R130A]
(Plasmid #221197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21-DE3 Rosetta Star cells can be used for expression. grow at 37˚C then shift to 16˚C at OD600 one doubling before inducing with IPTG
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ScChd1 (aa118-1274)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3468
  • Mutation
    R126A, R130A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-tag (Precission protease cleavable) (N terminal on backbone)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer 5’ caa tct aca gtg aaa ata cca aca GCg ttt tct aat GCt caa aac aaa acg gta aat tat aac
  • 3′ sequencing primer 5’ gttataatttaccgttttgttttgaGCattagaaaacGCtgttggtattttcactgtagattg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-ScChd1[R126A/R130A] was a gift from Gregory Bowman (Addgene plasmid # 221197 ; http://n2t.net/addgene:221197 ; RRID:Addgene_221197)

  • For your References section:

    Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state. Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP. Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16. 10.1038/s41594-021-00719-x PubMed 35173352
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