pGEM-601[insert(+1) TA-rich]
(Plasmid
#221196)
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Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM3z
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2743
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Modifications to backboneinsertion of 601 sequence
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name601[insert(+1)TA-rich]
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SpeciesSynthetic
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Mutation601 has an additional mutation added 22 bp from dyad on TA-rich side
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byoriginal pGEM-601 was a gift from Blaine Bartholomew and Jon Widom Labs
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-601[insert(+1) TA-rich] was a gift from Gregory Bowman (Addgene plasmid # 221196 ; http://n2t.net/addgene:221196 ; RRID:Addgene_221196) -
For your References section:
Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state. Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP. Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16. 10.1038/s41594-021-00719-x PubMed 35173352