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Addgene

pGEM-601[insert(+1) TA-rich]
(Plasmid #221196)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221196 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM3z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2743
  • Modifications to backbone
    insertion of 601 sequence
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    601[insert(+1)TA-rich]
  • Species
    Synthetic
  • Mutation
    601 has an additional mutation added 22 bp from dyad on TA-rich side

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    original pGEM-601 was a gift from Blaine Bartholomew and Jon Widom Labs

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-601[insert(+1) TA-rich] was a gift from Gregory Bowman (Addgene plasmid # 221196 ; http://n2t.net/addgene:221196 ; RRID:Addgene_221196)
  • For your References section:

    Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state. Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP. Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16. 10.1038/s41594-021-00719-x PubMed 35173352