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pTE5419_p3WJdB-glcO36
(Plasmid #221193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTE5417
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7 promoter-glcO36
  • Alt name
    GlcR sensor template
  • Species
    Synthetic
  • Insert Size (bp)
    68
  • Promoter T7 promoter with downstream glcO36 operator

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer CGGTTCCTGGCCTTTTGC
  • 3′ sequencing primer GATAGGTGCCTCACTGATTAAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.26.591264 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE5419_p3WJdB-glcO36 was a gift from Tobias Erb (Addgene plasmid # 221193 ; http://n2t.net/addgene:221193 ; RRID:Addgene_221193)

  • For your References section:

    In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade. Barthel S, Brenker L, Diehl C, Bohra N, Giaveri S, Paczia N, Erb TJ. Synthetic Biology, 2024;, ysae013 10.1093/synbio/ysae013
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