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pTE5418_pMBP-GlcR
(Plasmid #221192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTE5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli BL21 AI works great as expression strain
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    glcR (pden4400 from Paracoccus denitrificans)
  • Species
    Paracoccus denitrificans
  • Insert Size (bp)
    847
  • GenBank ID
    ABL72464.1
  • Promoter T7 promoter-lacO
  • Tag / Fusion Protein
    • 10x His tag with E. coli maltose binding protein (MBP) (N terminal on backbone)

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer GGTCGTCAGACTGTCGATG
  • 3′ sequencing primer GAAGCCTGCATAACGCGAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.26.591264 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE5418_pMBP-GlcR was a gift from Tobias Erb (Addgene plasmid # 221192 ; http://n2t.net/addgene:221192 ; RRID:Addgene_221192)

  • For your References section:

    In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade. Barthel S, Brenker L, Diehl C, Bohra N, Giaveri S, Paczia N, Erb TJ. Synthetic Biology, 2024;, ysae013 10.1093/synbio/ysae013
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