pConRef-2H12
(Plasmid #221182)

• Purpose: cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
• Depositing Lab: Urs Jenal
• Publication: Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.

Backbone

• Vector backbone: p2H12
• Backbone manufacturer: Andreas Kaczmarczyk
• Backbone size w/o insert (bp): 2000
• Total vector size (bp): 4000
• Modifications to backbone: cdGreen and mScarlet-I replacing cdGreen; Ptet-TetR replaced by constitutive promoter
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: cdGreen
• Alt name: 2H12
• Species: Synthetic
• Insert Size (bp): 1300
• Promoter: Constitutive

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: 13378 (attttactagtTAGAAAAACAACTTAAATGTGAAAGTGGGTC)

Gene/Insert 2

• Gene/Insert name: mScarlet-I
• Species: Synthetic
• Insert Size (bp): 700
• Mutation: N-terminus changed: starts with MSKKYGEAVIKE ...
• Promoter: None (same as cdGreen)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pConRef-2H12.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry