pVETL-Cmcs-Flag-Ataxin3Q25
(Plasmid
#22118)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVETL-Cmcs
- Backbone size w/o insert (bp) 5756
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtaxin 3
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Entrez GeneATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVETL-Cmcs-Flag-Ataxin3Q25 was a gift from Henry Paulson (Addgene plasmid # 22118 ; http://n2t.net/addgene:22118 ; RRID:Addgene_22118) -
For your References section:
Cellular turnover of the polyglutamine disease protein ataxin-3 is regulated by its catalytic activity. Todi SV, Laco MN, Winborn BJ, Travis SM, Wen HM, Paulson HL. J Biol Chem. 2007 Oct 5. 282(40):29348-58. 10.1074/jbc.M704126200 PubMed 17693639