pGEX-6P1-Ataxin3Q22-UIM*
(Plasmid #22114)

• Depositing Lab: Henry Paulson
• Publication: Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-43.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ataxin 3
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: UIM 1, 2, 3 SA-DG
• Entrez Gene: ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry