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Addgene

pHelper-CRISPRi-sgRNA
(Plasmid #221135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech/Takara
  • Total vector size (bp) 4924
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA-scaffold with dCas9 handle
  • Species
    Synthetic
  • Promoter J23119(SpeI)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

XhoI/NotI and EcoRI sites for subcloning of sgRNA-encoding region to pCB-CRISPRi (Addgene plasmid #221136). Plasmid alias: pSAST163.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper-CRISPRi-sgRNA was a gift from Craig Roy (Addgene plasmid # 221135 ; http://n2t.net/addgene:221135 ; RRID:Addgene_221135)

  • For your References section:

    CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii. Steiner S, Roy CR. mSphere. 2024 Nov 19:e0052324. doi: 10.1128/msphere.00523-24. 10.1128/msphere.00523-24 PubMed 39560384
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