pQC
(Plasmid #221134)

• Purpose: Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator
• Depositing Lab: Daniel Daley
• Publication: Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.1021/acssynbio.3c00720.

Backbone

• Vector backbone: pSEVA631
• Backbone manufacturer: Standard European Vector Architecture
• Total vector size (bp): 5046
• Modifications to backbone: contains a resistance cassette that confers resistance to Spectinomycin (50 ug/mL)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: [PibpA-gfpASV]
• Species: Synthetic
• Promoter: ibpA

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: [PcpxPmcherryASV] OPT
• Species: Synthetic
• Promoter: cpxP

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry