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Addgene

pQC
(Plasmid #221134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA631
  • Backbone manufacturer
    Standard European Vector Architecture
  • Total vector size (bp) 5046
  • Modifications to backbone
    contains a resistance cassette that confers resistance to Spectinomycin (50 ug/mL)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    [PibpA-gfpASV]
  • Species
    Synthetic
  • Promoter ibpA

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    [PcpxPmcherryASV] OPT
  • Species
    Synthetic
  • Promoter cpxP

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQC was a gift from Daniel Daley (Addgene plasmid # 221134 ; http://n2t.net/addgene:221134 ; RRID:Addgene_221134)

  • For your References section:

    Biosensor that Detects Stress Caused by Periplasmic Proteins. Cumming AJ, Khananisho D, Balka M, Liljestrand N, Daley DO. ACS Synth Biol. 2024 Apr 27. doi: 10.1021/acssynbio.3c00720. 10.1021/acssynbio.3c00720 PubMed 38676700
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