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pYFAC-CH2-ubi-M-pyrG
(Plasmid #221132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYFAC-ubi-M-CH2
  • Backbone size (bp) 15795
  • Modifications to backbone
    Contains autotrophy marker pyrG fused to a ubi-M degron for more stringent plasmid selection
  • Vector type
    Synthetic Biology ; Expression in Aspergillus and related filamentous fungi
  • Promoter PalcA-T1-PalcS/M-T2-PaldA expression cassette (alcohol inducible)
  • Selectable markers
    URA3 ; ubi-M-pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For improved transformation efficiency, use a DH10B E coli, and electroporation.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from pYFAC-CH2 (Addgene Plasmid #168978), incorporating ubi-M in the N-terminal of pyrG for more stringent selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFAC-CH2-ubi-M-pyrG was a gift from Yit Heng Chooi (Addgene plasmid # 221132 ; http://n2t.net/addgene:221132 ; RRID:Addgene_221132)

  • For your References section:

    Next-generation AMA1-based plasmids for enhanced heterologous expression in filamentous fungi. Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, Chooi YH. Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 10.1111/1751-7915.70010. 10.1111/1751-7915.70010 PubMed 39276061
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