pDP0437
(Plasmid #221127)

• Purpose: Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX
• Depositing Lab: Allyson O’Donnell
• Publication: Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. Epub 2024 May 29.

Backbone

• Vector backbone: pFA6-HPHMX4
• Vector type: Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pHluorin
• Species: S. cerevisiae (budding yeast)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This pHluorin integration plasmid was constructed by subcloning the 977 bp SalI/BglII fragment from pBW1571 (pFA6a-pHluorin::KANMX6 from (Prosser et al., 2010) into the SalI/BglII sites of pAG32 (pFA6-HPHMX4 described in (Goldstein and McCusker, 1999). The resulting plasmid was used for C-terminal tagging of the endogenous STE3 locus using a PCR-based integration strategy described in (Longtine et al., 1998)