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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST
(Plasmid #221118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MFA-IgKappa-FAPoptim-STE3-deltaPEST
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    STE3 mutant that lacks the PEST domain in the C-terminal tail (amino acids 413-470)
  • Promoter STE3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino acid 413 was mutated to a stop codon using site-directed mutagenesis, creating a truncation mutant of Ste3s C-tail at this residue in the pRS415-STE3pr-MFA-Igkappa-FAP-STE3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST was a gift from Allyson O’Donnell (Addgene plasmid # 221118 ; http://n2t.net/addgene:221118 ; RRID:Addgene_221118)

  • For your References section:

    Optimization of the fluorogen-activating protein tag for quantitative protein trafficking and colocalization studies in S. cerevisiae. Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF. Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. Epub 2024 May 29. 10.1091/mbc.E24-04-0174 PubMed 38809589
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