pTomatoSlicer
(Plasmid
#221085)
-
Purpose(Empty Backbone) Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneUnknown
-
Modifications to backboneContains eGFP-IRES (from Aequorea victoria) and tdTomato.
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTomatoSlicer was a gift from Chaolin Zhang (Addgene plasmid # 221085 ; http://n2t.net/addgene:221085 ; RRID:Addgene_221085) -
For your References section:
CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements. Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu Q, Zhang C. Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8. 10.1038/s41467-024-47140-8 PubMed 38714659
