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pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
(Plasmid #221082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Amp backbone
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACC-msfGFP[r5M]_ACC-mEBFP2
  • Species
    Synthetic
  • Promoter CMV, T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a polycistronic expression insert. It is used for IVT mRNA production. AG dinucleotide may be needed depending on capping technology following T7 promoter. Please visit https://doi.org/10.1101/2023.05.26.541240 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2 was a gift from Mikhail Shapiro (Addgene plasmid # 221082 ; http://n2t.net/addgene:221082 ; RRID:Addgene_221082)

  • For your References section:

    SEMPER: Stoichiometric expression of mRNA polycistrons by eukaryotic ribosomes for compact, ratio-tunable multi-gene expression. Duan M, Dev I, Lu A, Ayrapetyan G, You MY, Shapiro MG. Cell Syst. 2024 Jul 17;15(7):597-609.e4. doi: 10.1016/j.cels.2024.06.001. Epub 2024 Jul 5. 10.1016/j.cels.2024.06.001 PubMed 38971149
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