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pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
(Plasmid #221080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV Sport
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
  • Species
    Anabaena flos-aquae
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a polycistronic expression insert. Please visit https://doi.org/10.1101/2023.05.26.541240 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry was a gift from Mikhail Shapiro (Addgene plasmid # 221080 ; http://n2t.net/addgene:221080 ; RRID:Addgene_221080)

  • For your References section:

    SEMPER: Stoichiometric expression of mRNA polycistrons by eukaryotic ribosomes for compact, ratio-tunable multi-gene expression. Duan M, Dev I, Lu A, Ayrapetyan G, You MY, Shapiro MG. Cell Syst. 2024 Jul 17;15(7):597-609.e4. doi: 10.1016/j.cels.2024.06.001. Epub 2024 Jul 5. 10.1016/j.cels.2024.06.001 PubMed 38971149
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