pT/Caggs-NRAS G12V-IRES-ASK1
(Plasmid
#221074)
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PurposeExpresses NRAS G12V and ASK1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT/Caggs
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameNRAS
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SpeciesH. sapiens (human)
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MutationG12V
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Entrez GeneNRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
Gene/Insert 2
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Gene/Insert nameASK1
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Alt nameapoptosis signal-regulating kinase 1
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SpeciesM. musculus (mouse)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOhlfest Lab (Addgene Plasmid #20205)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT/Caggs-NRAS G12V-IRES-ASK1 was a gift from Hidenori Ichijo (Addgene plasmid # 221074 ; http://n2t.net/addgene:221074 ; RRID:Addgene_221074) -
For your References section:
Apoptosis signal-regulating kinase 1 promotes inflammation in senescence and aging. Takeru Odawara, Shota Yamauchi, Hidenori Ichijo. Communications Biology volume 7, Article number: 691 10.1038/s42003-024-06386-0