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Addgene

pWJPrP34
(Plasmid #22107)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBTIIKS(+)
  • Backbone size w/o insert (bp) 2900
  • Vector type
    construction of targeting vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PrP exon 3 EcoR1 fragment
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    Added a Cla1 site right before translation start and Eag1 downstream of translation stop site.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Melton; Edinburgh, Scotland

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWJPrP34 was a gift from Susan Lindquist (Addgene plasmid # 22107 ; http://n2t.net/addgene:22107 ; RRID:Addgene_22107)

  • For your References section:

    Spontaneous generation of prion infectivity in fatal familial insomnia knockin mice. Jackson WS, Borkowski AW, Faas H, Steele AD, King OD, Watson N, Jasanoff A, Lindquist S. Neuron. 2009 Aug 27. 63(4):438-50. 10.1016/j.neuron.2009.07.026 PubMed 19709627
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