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Addgene

pAAV-MCHpr-GCaMP6f
(Plasmid #221039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression, AAV ; Rat Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    1350
  • Mutation
    A317E
  • Promoter Rat MCH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-MCHpr-GCaMP6f was a gift from Yanhua Huang (Addgene plasmid # 221039 ; http://n2t.net/addgene:221039 ; RRID:Addgene_221039)

  • For your References section:

    Rapid Eye Movement Sleep Engages Melanin-Concentrating Hormone Neurons to Reduce Cocaine Seeking. Guo R, Wang Y, Yan R, Chen B, Ding W, Gorczyca MT, Ozsoy S, Cai L, Hines RL, Tseng GC, Allocca G, Dong Y, Fang J, Huang YH. Biol Psychiatry. 2022 Dec 1;92(11):880-894. doi: 10.1016/j.biopsych.2022.06.006. Epub 2022 Jun 13. 10.1016/j.biopsych.2022.06.006 PubMed 35953320
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