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pAAV-MCHpr-DREADD (Gq)-mCherry
(Plasmid #221036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, AAV ; Rat Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DREADD Gq-mCherry
  • Alt name
    hM3Dq-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    2500
  • Promoter Rat MCH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BsrGi (not destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-MCHpr-DREADD (Gq)-mCherry was a gift from Yanhua Huang (Addgene plasmid # 221036 ; http://n2t.net/addgene:221036 ; RRID:Addgene_221036)

  • For your References section:

    Cocaine-induced neural adaptations in the lateral hypothalamic melanin-concentrating hormone neurons and the role in regulating rapid eye movement sleep after withdrawal. Wang Y, Guo R, Chen B, Rahman T, Cai L, Li Y, Dong Y, Tseng GC, Fang J, Seney ML, Huang YH. Mol Psychiatry. 2021 Jul;26(7):3152-3168. doi: 10.1038/s41380-020-00921-1. Epub 2020 Oct 22. 10.1038/s41380-020-00921-1 PubMed 33093653
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