pAAV-MCHpr-DREADD (Gq)-mCherry
(Plasmid #221036)

• Purpose: Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter
• Depositing Lab: Yanhua Huang
• Publication: Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168. doi: 10.1038/s41380-020-00921-1. Epub 2020 Oct 22.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression, AAV ; Rat Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DREADD Gq-mCherry
• Alt name: hM3Dq-mCherry
• Species: Synthetic
• Insert Size (bp): 2500
• Promoter: Rat MCH

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BsrGi (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry