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Addgene

pMMS22L-siR3
(Plasmid #221013)

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Item Catalog # Description Quantity Price (USD)
Plasmid 221013 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV6-Entry
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 4880
  • Total vector size (bp) 8610
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    CopyCutter
  • Growth instructions
    EXTREMELY UNSTABLE IN BACTERIA. Must be propagated in CopyCutter EPI400 E. coli cells. See additional instructions. It is recommended to sequence entire ORF after each prep.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MMS22L
  • Alt name
    C16orf167
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3730
  • Mutation
    silent mutations MMS22L: 5'-aaAacGtgTtgCtgTgaC-3' to confer siRNA resistance
  • GenBank ID
    NM_198468.2
  • Entrez Gene
    MMS22L (a.k.a. C6orf167, dJ39B17.2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMS22L-siR3 was a gift from Daniel Durocher (Addgene plasmid # 221013 ; http://n2t.net/addgene:221013 ; RRID:Addgene_221013)

  • For your References section:

    The MMS22L-TONSL complex mediates recovery from replication stress and homologous recombination. O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, Durocher D. Mol Cell. 2010 Nov 24;40(4):619-31. doi: 10.1016/j.molcel.2010.10.024. Epub 2010 Nov 4. 10.1016/j.molcel.2010.10.024 PubMed 21055983
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