SMN2E7-Fluc-Nluc-reporter
(Plasmid
#221005)
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PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFluc-SMN2E7-STOP-Nluc
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made bySMN2 exon 7 and surrounding sequences cloned from pCI-SMN2 (Addgene #72287)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SMN2E7-Fluc-Nluc-reporter was a gift from Benjamin Blencowe & Mikko Taipale (Addgene plasmid # 221005 ; http://n2t.net/addgene:221005 ; RRID:Addgene_221005) -
For your References section:
Efficient, specific, and combinatorial control of endogenous exon splicing with dCasRx-RBM25. Li JD, Taipale M, Blencowe BJ. Mol Cell. 2024 Jul 11;84(13):2573-2589.e5. doi: 10.1016/j.molcel.2024.05.028. Epub 2024 Jun 24. 10.1016/j.molcel.2024.05.028 PubMed 38917795
