Skip to main content
Addgene

CMV-dCasRx-ccdB
(Plasmid #221003)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAC1801_pmax-dCasRx (Addgene #118634)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This was the destination vector used for generating the dCasRx-SF library for screening by co-transfection with the luciferase reporters. We have found that cloning dCasRx-SFs into the pXR002 backbone gave greater transfection efficiency for endogenous applications. We recommend using the EF1a-dCasRx-RBM25 vector (Addgene #221001) for endogenous targeting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-dCasRx-ccdB was a gift from Benjamin Blencowe & Mikko Taipale (Addgene plasmid # 221003 ; http://n2t.net/addgene:221003 ; RRID:Addgene_221003)

  • For your References section:

    Efficient, specific, and combinatorial control of endogenous exon splicing with dCasRx-RBM25. Li JD, Taipale M, Blencowe BJ. Mol Cell. 2024 Jul 11;84(13):2573-2589.e5. doi: 10.1016/j.molcel.2024.05.028. Epub 2024 Jun 24. 10.1016/j.molcel.2024.05.028 PubMed 38917795
Related items:
Commonly requested with: