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Addgene

Src(KD/YF)-SsrA(Y7I)-mCer3
(Plasmid #221000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Src, SsrA, mCerulean3
  • Species
    M. musculus (mouse)
  • Mutation
    D388R/Y529F in Src, Y7I in SsrA
  • Entrez Gene
    Src (a.k.a. pp60c-src)
  • Promoter pCMV
  • Tag / Fusion Protein
    • mCerulean3 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Src(KD/YF)-SsrA(Y7I)-mCer3 was a gift from Klaus Hahn (Addgene plasmid # 221000 ; http://n2t.net/addgene:221000 ; RRID:Addgene_221000)

  • For your References section:

    Biosensors based on peptide exposure show single molecule conformations in live cells. Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm JB, Lavis LD, Elston TC, Hahn KM. Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 10.1016/j.cell.2021.09.026. Epub 2021 Oct 11. 10.1016/j.cell.2021.09.026 PubMed 34637702
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