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pRS2 (HIF2a-pRetro-Super)
(Plasmid #22100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetro-Super
  • Backbone manufacturer
    NKI-AVL
  • Backbone size w/o insert (bp) 6349
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a or HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthethic oligonucleotide
  • Insert Size (bp)
    58
  • Mutation
    synthethic oligonucleotide encoding shRNA targeting human HIF2a mRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer MSCV reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS2 (HIF2a-pRetro-Super) was a gift from William Kaelin (Addgene plasmid # 22100 ; http://n2t.net/addgene:22100 ; RRID:Addgene_22100)

  • For your References section:

    Inhibition of HIF2alpha is sufficient to suppress pVHL-defective tumor growth. Kondo K, Kim WY, Lechpammer M, Kaelin WG. PLoS Biol. 2003 Dec . 1(3):E83. 10.1371/journal.pbio.0000083 PubMed 14691554
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