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pDY2262 hACTB atgRNA Paired Guide 2
(Plasmid #220992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6-pegRNA-GG-acceptor (Addgene #132777)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ACTB atgRNA
  • gRNA/shRNA sequence
    GGAGGGGAAGACGGCCCGGGgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcggcttgtcgacgacggcggtctccgtcgtcaggatcat
  • Species
    H. sapiens (human)
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDY2262 hACTB atgRNA Paired Guide 2 was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 220992 ; http://n2t.net/addgene:220992 ; RRID:Addgene_220992)

  • For your References section:

    Precise kilobase-scale genomic insertions in mammalian cells using PASTE. Fell CW, Schmitt-Ulms C, Tagliaferri DV, Gootenberg JS, Abudayyeh OO. Nat Protoc. 2024 Dec 15. doi: 10.1038/s41596-024-01090-z. 10.1038/s41596-024-01090-z PubMed 39676077
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