ORFtag_TetR-SD_Frame3
(Plasmid #220983)

• Purpose: ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
• Depositing Lab: Alexander Stark
• Publication: Proteome-scale tagging and functional screening in mammalian cells by ORFtag (unpublished)

Backbone

• Vector backbone: ORFtag backbone
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TetR repressor
• Alt name: TetR-splice donor in Frame3

Resource Information

• Supplemental Documents:
  • orftag_tetr-sd_frame3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry