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Addgene

pLenti6.3-SaCas9-BlastR
(Plasmid #220966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SaCas9

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.3-SaCas9-BlastR was a gift from Hyongbum Kim (Addgene plasmid # 220966 ; http://n2t.net/addgene:220966 ; RRID:Addgene_220966)

  • For your References section:

    Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s. Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho SR, Kim HH. Nat Methods. 2023 Jul;20(7):999-1009. doi: 10.1038/s41592-023-01875-2. Epub 2023 May 15. 10.1038/s41592-023-01875-2 PubMed 37188955
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