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Addgene

3CB1/cSprouty2
(Plasmid #22096)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sprouty 2
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1400
  • Entrez Gene
    SPRY2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Partial chicken Sprouty2 cDNA clone was isolated from a chick embryonic brain cDNA library (Lambda Zap). The entire coding region and 3' UTR sequences are included.

For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.4kb.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3CB1/cSprouty2 was a gift from Gail Martin (Addgene plasmid # 22096 ; http://n2t.net/addgene:22096 ; RRID:Addgene_22096)

  • For your References section:

    Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Minowada G, Jarvis LA, Chi CL, Neubuser A, Sun X, Hacohen N, Krasnow MA, Martin GR. Development. 1999 Oct . 126(20):4465-75. PubMed 10498682
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