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Addgene

OgeuIscB ωRNA-v2
(Plasmid #220958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
  • gRNA/shRNA sequence
    agagacgAAAAGAGTGAACGAGAtcgtctca

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OgeuIscB ωRNA-v2 was a gift from Weixin Tang (Addgene plasmid # 220958 ; http://n2t.net/addgene:220958 ; RRID:Addgene_220958)

  • For your References section:

    Assessing and engineering the IscB-omegaRNA system for programmed genome editing. Yan H, Tan X, Zou S, Sun Y, Ke A, Tang W. Nat Chem Biol. 2024 Jul 8. doi: 10.1038/s41589-024-01669-3. 10.1038/s41589-024-01669-3 PubMed 38977787
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