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Addgene

enOgeuIscB with ωRNA-v13
(Plasmid #220940)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS

Gene/Insert 2

  • Gene/Insert name
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    enOgeuIscB with ωRNA-v13 was a gift from Weixin Tang (Addgene plasmid # 220940 ; http://n2t.net/addgene:220940 ; RRID:Addgene_220940)

  • For your References section:

    Assessing and engineering the IscB-omegaRNA system for programmed genome editing. Yan H, Tan X, Zou S, Sun Y, Ke A, Tang W. Nat Chem Biol. 2024 Jul 8. doi: 10.1038/s41589-024-01669-3. 10.1038/s41589-024-01669-3 PubMed 38977787
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