pK-11
(Plasmid #22094)

• Depositing Lab: Gail Martin
• Publication: Meyers et al Nat Genet. 1998 Feb . 18(2):136-41.

Backbone

• Vector backbone: pBluescript SK
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Frt-PGKNeo-Frt-LoxP
• Insert Size (bp): 1900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FRT sequence (+strand) is 5'-GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC-3' and the LoxP sequence (+strand) is 5'-ATAACTTCGTATAGCATACATTATACGAAGTTAT-3'. pK11 is missing the polylinker sites between the 5' FRT sequence and the PGKNeo that are present in pM30.

Known unique sites include: SacI, SacII, SalI, BamHI, ApaI, KpnI and HindIII.